Journal: Bioactive Materials

Article Title: Immunomodulatory effects of biodegradable Mg–Cu–Zn alloy in esophageal cancer

doi: 10.1016/j.bioactmat.2026.02.046

Figure Lengend Snippet: Pure Mg, Mg–Cu, and Mg–Cu–Zn alloys inhibit tumor cell proliferation in immunocompetent C57BL/6 mice. (a) Representative H&E-stained tumor images showing necrosis and Ki-67 IHC staining in serial sections. Red dashed boxes indicate necrotic areas. (b) Quantification of tumor necrosis rates across groups. (c) Comparison of Ki-67 labeling index between perinecrotic and non-perinecrotic tumor cells. (d, e) Statistical analysis of Ki-67 labeling index in perinecrotic and non-perinecrotic regions across groups. (f) Representative IHC images of Ki-67 expression in viable tumor cells from perinecrotic and non-perinecrotic regions. Scale bar: 200 μm. p < 0.05 (∗), p < 0.001 (∗∗∗).

Article Snippet: Tissue sections were then incubated with primary antibodies against iNOS (22226-1-AP, ProteinTech, China), CD163 (A26411PM, Abclone, China), CD8 (SP16, Maixin, China), CD4 (SP35, Maixin, China) or Ki-67 (12202S, Cell Signaling Technology) for 12 h at 4 °C, followed by secondary antibodies (Beyotime Biotechnology, Nantong, China).

Techniques: Staining, Immunohistochemistry, Comparison, Labeling, Expressing

Journal: International Journal of Oncology

Article Title: Overcoming acquired doxorubicin resistance of ovarian carcinoma cells by verapamil-mediated promotion of DNA damage-driven cytotoxicity

doi: 10.3892/ijo.2026.5861

Figure Lengend Snippet: Cell cycle progression and proliferation following treatment of A2780 and A2780ADR cells with Topo II inhibitors. Logarithmically growing cells were treated with the indicated concentrations of Doxo or etoposide Eto for (A) 24 or (B) 72 h. Afterwards, cell cycle distribution was analyzed by flow cytometry and the percentage of cells present in different phases of the cell cycle (SubG 1 -, G1-, S- and G 2 /M-phase) was quantified. Data shown in the histogram (left panel) are the mean ± SD from n=3 independent experiments each performed in biological triplicates. The table on the right panel summarizes the mean values and indicates statistical differences between the individual groups. * P≤0.05; ** P≤0.01 (A2780 vs. A2780ADR); # P≤0.05; ## P≤0.01 (Con vs. treated group). (C) Logarithmically growing parental A2780 and Doxo resistant A2780ADR cells were treated with the indicated concentrations of Doxo (0.1 and 1.0 μ M) or Eto (1.0 and 10 μ M). At 24 h later the percentage of Ki-67 positive or pH3 positive cells was determined as described in methods. Total magnification, ×400. Quantitative data shown in the histogram are the mean ± SD from n=3 independent experiments, each performed with n=5 biological replicates. * P≤0.05; ** P≤0.01; **** P≤0.0001 (A2780 vs. A2780ADR). Con vs. treatment: # P≤0.05; ## P≤0.01; ### P≤0.001. (D) Logarithmically growing parental A2780 and Doxo resistant A2780ADR cells were treated with the indicated concentrations of Doxo (0.1 and 1.0 μ M) or Eto (1.0 and 10 μ M). At 24 h later cells were pulse-labeled with EdU for 2 h as described in Methods and the percentage of EdU positive cells was determined microscopically (total magnification, ×400). Quantitative data shown in the histogram are the mean ± SD from five biological replicates. A2780 as compared with A2780ADR: ** P≤0.05; **** P≤0.0001. Con vs. treatment: ## P≤0.01; ### P≤0.001. Doxo, doxorubicin; Eto, etoposide; SD, standard deviation.

Article Snippet: The following primary antibodies were used: Copper transporting ATPase (ATP7A), extracellular regulated kinase 2 (ERK2), phosphorylated (p)-histone H3 (Ser10) from Thermo Fisher Scientific Inc., cleaved caspase-7 (Asp198), p-Chk1 (Ser 345), cyclin B1, galactosidase β (E2U2I), GAPDH (14C10), MDR1/ABCB1 (D3H1Q), p-P53 (S15), PARP, TopBP1(D8G4L), topoisomerase IIa (D10G9), 53BP1 and Ki67 were from Cell Signaling Technology Inc., pChk2 (T68) [Y171], copper uptake protein 1 (CTR1/SLC31A1) [EPR7936] and Rad51 from Abcam, γH2AX (Ser 139) clone JBW301, p-KAP-1 (S824) and p-RPA32 (S4/S8) from Bethyl Laboratories Inc., organic cation transporter-2 (OCT2) from Biozol Diagnostics Vertrieb GmbH, p16 (F-12) and p21 (C-19) from Santa Cruz Biotechnology, Inc. As secondary antibodies, horseradish peroxidase-conjugated secondary antibodies goat anti-mouse IgG and mouse anti-rabbit IgG were used (Rockland Immunochemicals Inc.).

Techniques: Flow Cytometry, Labeling, Standard Deviation