Journal: Non-coding RNA Research

Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma

doi: 10.1016/j.ncrna.2026.03.003

Figure Lengend Snippet: circSMAD4 depletion in macrophages restrains LUAD growth and metastasis in vivo. (A) Schematic of orthotopic lung implantation and experimental metastasis models using LLC cells mixed with BMDMs expressing shNC or sh-circSMAD4. (B) Representative images of orthotopic lung tumors. (C) Tumor weight of orthotopic implants. (D) Overall survival of mice bearing orthotopic tumors. (E) Immunofluorescence showing F4/80 and circSMAD4 signals in tumor tissues. Scale bar, 50 μm. (F, G) Representative Ki-67 IHC staining and quantification in orthotopic tumors. Scale bar, 50 μm. (H) Representative bioluminescence images of lung tumor burden in the metastasis model. (I) Tumor weight in the metastasis model. (J) Overall survival of mice in the metastasis model. (K–M) Representative IHC staining and quantification of E-cadherin and vimentin in tumors. Scale bar, 50 μm. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001; ns, not significant.

Article Snippet: Sections were incubated with primary antibodies against Ki-67 (Servicebio, Cat# GB111499 ), E-cadherin (Proteintech, Cat# 20874-1-AP), and Vimentin (Proteintech, Cat# 10366-1-AP).

Techniques: In Vivo, Expressing, Immunofluorescence, Immunohistochemistry

Journal: Bioactive Materials

Article Title: Immunomodulatory effects of biodegradable Mg–Cu–Zn alloy in esophageal cancer

doi: 10.1016/j.bioactmat.2026.02.046

Figure Lengend Snippet: Pure Mg, Mg–Cu, and Mg–Cu–Zn alloys inhibit tumor cell proliferation in immunocompetent C57BL/6 mice. (a) Representative H&E-stained tumor images showing necrosis and Ki-67 IHC staining in serial sections. Red dashed boxes indicate necrotic areas. (b) Quantification of tumor necrosis rates across groups. (c) Comparison of Ki-67 labeling index between perinecrotic and non-perinecrotic tumor cells. (d, e) Statistical analysis of Ki-67 labeling index in perinecrotic and non-perinecrotic regions across groups. (f) Representative IHC images of Ki-67 expression in viable tumor cells from perinecrotic and non-perinecrotic regions. Scale bar: 200 μm. p < 0.05 (∗), p < 0.001 (∗∗∗).

Article Snippet: Tissue sections were then incubated with primary antibodies against iNOS (22226-1-AP, ProteinTech, China), CD163 (A26411PM, Abclone, China), CD8 (SP16, Maixin, China), CD4 (SP35, Maixin, China) or Ki-67 (12202S, Cell Signaling Technology) for 12 h at 4 °C, followed by secondary antibodies (Beyotime Biotechnology, Nantong, China).

Techniques: Staining, Immunohistochemistry, Comparison, Labeling, Expressing

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Tebuconazole Toxicity in the Parotid Gland of Adolescent Rats

doi: 10.1002/jbt.70833

Figure Lengend Snippet: Mean ± SD of the total number of Ki‐67 labeled nuclei in 10 fields in parotid salivary glands in Wistar rats exposed to tebuconazole ( n = 5).

Article Snippet: To perform this technique, the protocol in Moretti et al. [ ] was used to evaluate markers of cell proliferation (Ki‐67) (Biocare Medical, USA—1:150), apoptosis (cleaved caspase‐3), oxidative stress (8‐hydroxy‐2′‐deoxyguanosine—8‐OHdG), epithelial remodeling (cytokeratin 7—CK7) (Santa Cruz Biotechnology, USA—1:500), and inflammation (cyclooxygenase‐2—COX‐2) (Abcam—1:100).

Techniques: Labeling

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Tebuconazole Toxicity in the Parotid Gland of Adolescent Rats

doi: 10.1002/jbt.70833

Figure Lengend Snippet: Photomicrograph of Ki‐67 immunostaining in parotid salivary glands of Wistar rats exposed to tebuconazole, in the following groups: (A) Control, (B) 10 mg/kg group, (C) 20 mg/kg group, and (D) 50‐mg/kg. The arrows indicate marked cores. Scale bar = 50 μm.

Article Snippet: To perform this technique, the protocol in Moretti et al. [ ] was used to evaluate markers of cell proliferation (Ki‐67) (Biocare Medical, USA—1:150), apoptosis (cleaved caspase‐3), oxidative stress (8‐hydroxy‐2′‐deoxyguanosine—8‐OHdG), epithelial remodeling (cytokeratin 7—CK7) (Santa Cruz Biotechnology, USA—1:500), and inflammation (cyclooxygenase‐2—COX‐2) (Abcam—1:100).

Techniques: Immunostaining, Control